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Dengan Undang-Undang Nomor 7 Tahun 1994 tentang Pengesahan Agreement Establishing The World Trade Organization (Persetujuan Pembentukan Organisasi Perdagangan Dunia) Indonesia telah secara resmi sebagai salah satu anggota Organisasi Perdagangan Dunia (World Trade Organization - WTO). TRIPs (Trade Related Aspects Of Intellectual Properti Raigt, including Trade in Counter Goods - Aspek-aspek Dagang yang terkait dengan hak-hak Atas kekayaan Intelektual, termasuk Perdagangan Barang Palsu) merupakan salah satu persetujuan yang berada dibawah pengelolaan organisasi perdagangan tersebut. Menurut Rooseno Harjowidigjo (1994) HAKI dalam TRIPs itu antara lain terdiri dari hak cipta dan Hak-Hak yang terkait, merek Dagang, Indikasi Geografis, Desain Industri, Paten, Desain Tata letak (Topografi) Sirkuit Terpadu, dan Perlindungan Informasi yang dirahasiakan.

Dengan Undang-Undang Nomor 7 Tahun 1994 tentang Pengesahan Agreement Establishing The World Trade Organization (Persetujuan Pembentukan Organisasi Perdagangan Dunia) Indonesia telah secara resmi sebagai salah satu anggota Organisasi Perdagangan Dunia (World Trade Organization - WTO). TRIPs (Trade Related Aspects Of Intellectual Properti Raigt, including Trade in Counter Goods - Aspek-aspek Dagang yang terkait dengan hak-hak Atas kekayaan Intelektual, termasuk Perdagangan Barang Palsu) merupakan salah satu persetujuan yang berada dibawah pengelolaan organisasi perdagangan tersebut. Menurut Rooseno Harjowidigjo (1994) HAKI dalam TRIPs itu antara lain terdiri dari hak cipta dan Hak-Hak yang terkait, merek Dagang, Indikasi Geografis, Desain Industri, Paten, Desain Tata letak (Topografi) Sirkuit Terpadu, dan Perlindungan Informasi yang dirahasiakan.

Dengan Undang-Undang Nomor 7 Tahun 1994 tentang Pengesahan Agreement Establishing The World Trade Organization (Persetujuan Pembentukan Organisasi Perdagangan Dunia) Indonesia telah secara resmi sebagai salah satu anggota Organisasi Perdagangan Dunia (World Trade Organization - WTO). TRIPs (Trade Related Aspects Of Intellectual Properti Raigt, including Trade in Counter Goods - Aspek-aspek Dagang yang terkait dengan hak-hak Atas kekayaan Intelektual, termasuk Perdagangan Barang Palsu) merupakan salah satu persetujuan yang berada dibawah pengelolaan organisasi perdagangan tersebut. Menurut Rooseno Harjowidigjo (1994) HAKI dalam TRIPs itu antara lain terdiri dari hak cipta dan Hak-Hak yang terkait, merek Dagang, Indikasi Geografis, Desain Industri, Paten, Desain Tata letak (Topografi) Sirkuit Terpadu, dan Perlindungan Informasi yang dirahasiakan.

Dengan Undang-Undang Nomor 7 Tahun 1994 tentang Pengesahan Agreement Establishing The World Trade Organization (Persetujuan Pembentukan Organisasi Perdagangan Dunia) Indonesia telah secara resmi sebagai salah satu anggota Organisasi Perdagangan Dunia (World Trade Organization - WTO). TRIPs (Trade Related Aspects Of Intellectual Properti Raigt, including Trade in Counter Goods - Aspek-aspek Dagang yang terkait dengan hak-hak Atas kekayaan Intelektual, termasuk Perdagangan Barang Palsu) merupakan salah satu persetujuan yang berada dibawah pengelolaan organisasi perdagangan tersebut. Menurut Rooseno Harjowidigjo (1994) HAKI dalam TRIPs itu antara lain terdiri dari hak cipta dan Hak-Hak yang terkait, merek Dagang, Indikasi Geografis, Desain Industri, Paten, Desain Tata letak (Topografi) Sirkuit Terpadu, dan Perlindungan Informasi yang dirahasiakan.

Dengan Undang-Undang Nomor 7 Tahun 1994 tentang Pengesahan Agreement Establishing The World Trade Organization (Persetujuan Pembentukan Organisasi Perdagangan Dunia) Indonesia telah secara resmi sebagai salah satu anggota Organisasi Perdagangan Dunia (World Trade Organization - WTO). TRIPs (Trade Related Aspects Of Intellectual Properti Raigt, including Trade in Counter Goods - Aspek-aspek Dagang yang terkait dengan hak-hak Atas kekayaan Intelektual, termasuk Perdagangan Barang Palsu) merupakan salah satu persetujuan yang berada dibawah pengelolaan organisasi perdagangan tersebut. Menurut Rooseno Harjowidigjo (1994) HAKI dalam TRIPs itu antara lain terdiri dari hak cipta dan Hak-Hak yang terkait, merek Dagang, Indikasi Geografis, Desain Industri, Paten, Desain Tata letak (Topografi) Sirkuit Terpadu, dan Perlindungan Informasi yang dirahasiakan.

Dengan Undang-Undang Nomor 7 Tahun 1994 tentang Pengesahan Agreement Establishing The World Trade Organization (Persetujuan Pembentukan Organisasi Perdagangan Dunia) Indonesia telah secara resmi sebagai salah satu anggota Organisasi Perdagangan Dunia (World Trade Organization - WTO). TRIPs (Trade Related Aspects Of Intellectual Properti Raigt, including Trade in Counter Goods - Aspek-aspek Dagang yang terkait dengan hak-hak Atas kekayaan Intelektual, termasuk Perdagangan Barang Palsu) merupakan salah satu persetujuan yang berada dibawah pengelolaan organisasi perdagangan tersebut. Menurut Rooseno Harjowidigjo (1994) HAKI dalam TRIPs itu antara lain terdiri dari hak cipta dan Hak-Hak yang terkait, merek Dagang, Indikasi Geografis, Desain Industri, Paten, Desain Tata letak (Topografi) Sirkuit Terpadu, dan Perlindungan Informasi yang dirahasiakan.

Dengan Undang-Undang Nomor 7 Tahun 1994 tentang Pengesahan Agreement Establishing The World Trade Organization (Persetujuan Pembentukan Organisasi Perdagangan Dunia) Indonesia telah secara resmi sebagai salah satu anggota Organisasi Perdagangan Dunia (World Trade Organization - WTO). TRIPs (Trade Related Aspects Of Intellectual Properti Raigt, including Trade in Counter Goods - Aspek-aspek Dagang yang terkait dengan hak-hak Atas kekayaan Intelektual, termasuk Perdagangan Barang Palsu) merupakan salah satu persetujuan yang berada dibawah pengelolaan organisasi perdagangan tersebut. Menurut Rooseno Harjowidigjo (1994) HAKI dalam TRIPs itu antara lain terdiri dari hak cipta dan Hak-Hak yang terkait, merek Dagang, Indikasi Geografis, Desain Industri, Paten, Desain Tata letak (Topografi) Sirkuit Terpadu, dan Perlindungan Informasi yang dirahasiakan.

Dengan Undang-Undang Nomor 7 Tahun 1994 tentang Pengesahan Agreement Establishing The World Trade Organization (Persetujuan Pembentukan Organisasi Perdagangan Dunia) Indonesia telah secara resmi sebagai salah satu anggota Organisasi Perdagangan Dunia (World Trade Organization - WTO). TRIPs (Trade Related Aspects Of Intellectual Properti Raigt, including Trade in Counter Goods - Aspek-aspek Dagang yang terkait dengan hak-hak Atas kekayaan Intelektual, termasuk Perdagangan Barang Palsu) merupakan salah satu persetujuan yang berada dibawah pengelolaan organisasi perdagangan tersebut. Menurut Rooseno Harjowidigjo (1994) HAKI dalam TRIPs itu antara lain terdiri dari hak cipta dan Hak-Hak yang terkait, merek Dagang, Indikasi Geografis, Desain Industri, Paten, Desain Tata letak (Topografi) Sirkuit Terpadu, dan Perlindungan Informasi yang dirahasiakan.

Dengan Undang-Undang Nomor 7 Tahun 1994 tentang Pengesahan Agreement Establishing The World Trade Organization (Persetujuan Pembentukan Organisasi Perdagangan Dunia) Indonesia telah secara resmi sebagai salah satu anggota Organisasi Perdagangan Dunia (World Trade Organization - WTO). TRIPs (Trade Related Aspects Of Intellectual Properti Raigt, including Trade in Counter Goods - Aspek-aspek Dagang yang terkait dengan hak-hak Atas kekayaan Intelektual, termasuk Perdagangan Barang Palsu) merupakan salah satu persetujuan yang berada dibawah pengelolaan organisasi perdagangan tersebut. Menurut Rooseno Harjowidigjo (1994) HAKI dalam TRIPs itu antara lain terdiri dari hak cipta dan Hak-Hak yang terkait, merek Dagang, Indikasi Geografis, Desain Industri, Paten, Desain Tata letak (Topografi) Sirkuit Terpadu, dan Perlindungan Informasi yang dirahasiakan.

Dengan Undang-Undang Nomor 7 Tahun 1994 tentang Pengesahan Agreement Establishing The World Trade Organization (Persetujuan Pembentukan Organisasi Perdagangan Dunia) Indonesia telah secara resmi sebagai salah satu anggota Organisasi Perdagangan Dunia (World Trade Organization - WTO). TRIPs (Trade Related Aspects Of Intellectual Properti Raigt, including Trade in Counter Goods - Aspek-aspek Dagang yang terkait dengan hak-hak Atas kekayaan Intelektual, termasuk Perdagangan Barang Palsu) merupakan salah satu persetujuan yang berada dibawah pengelolaan organisasi perdagangan tersebut. Menurut Rooseno Harjowidigjo (1994) HAKI dalam TRIPs itu antara lain terdiri dari hak cipta dan Hak-Hak yang terkait, merek Dagang, Indikasi Geografis, Desain Industri, Paten, Desain Tata letak (Topografi) Sirkuit Terpadu, dan Perlindungan Informasi yang dirahasiakan.

Seaweed is one of the natural ingredients that have a variety of contents and sources of secondary metabolite compounds that have toxic activity. Toxic activity derived from a substance can be further developed and will provide benefits in the field of food and pharmacology. The presence of seaweed Glacilaria sp. and Gelidium sp. in the coastal area of Kalianda waters, South Lampung is quite abundant so that due to the availability of the abundance of these materials can be made of great potential to be utilized as a medicine. The purpose of this study was to analyze the level of toxicity using stratified estraction with n-hexane and methanol solvents and analyze chemical components with GC-MS based on the highest level of toxicity. The study was conducted from November to December 2021. Samples of Glacilaria sp. and Gelidium sp. taken from the waters of Kalianda, South Lampung. Furthermore, the samples were tested for toxicity by Brine Shrimp Lethality Test, phytochemical analysis, and GC-MS analysis. Based on the toxicity test results, the value of lc50 estrak n-hexane Glacilaria sp. 8446 µg/mL, methanol Glacilaria sp. 694 µg/mL n-hexane Gelidium sp. µg/mL and methanol gelidium sp. it amounted to 339 µg/mL. Phytochemical test results contain alkaloids, flavonoids, steroids and saponins while GC-MS test results contain steroids, flavonoids and diterpenoids. Differences in the level of toxicity of n-hexane and methanol in sebabakan by the nature of the compound so that the toxicity of methanol estrak Gelidium sp. higher. Differences in phytochemical test results and GC-MS are based on the percentage level of the compound in the material, so it is likely that compounds with a small percentage will not be detected in the phytochemical test.

Seaweed is one of the natural ingredients that have a variety of contents and sources of secondary metabolite compounds that have toxic activity. Toxic activity derived from a substance can be further developed and will provide benefits in the field of food and pharmacology. The presence of seaweed Glacilaria sp. and Gelidium sp. in the coastal area of Kalianda waters, South Lampung is quite abundant so that due to the availability of the abundance of these materials can be made of great potential to be utilized as a medicine. The purpose of this study was to analyze the level of toxicity using stratified estraction with n-hexane and methanol solvents and analyze chemical components with GC-MS based on the highest level of toxicity. The study was conducted from November to December 2021. Samples of Glacilaria sp. and Gelidium sp. taken from the waters of Kalianda, South Lampung. Furthermore, the samples were tested for toxicity by Brine Shrimp Lethality Test, phytochemical analysis, and GC-MS analysis. Based on the toxicity test results, the value of lc50 estrak n-hexane Glacilaria sp. 8446 µg/mL, methanol Glacilaria sp. 694 µg/mL n-hexane Gelidium sp. µg/mL and methanol gelidium sp. it amounted to 339 µg/mL. Phytochemical test results contain alkaloids, flavonoids, steroids and saponins while GC-MS test results contain steroids, flavonoids and diterpenoids. Differences in the level of toxicity of n-hexane and methanol in sebabakan by the nature of the compound so that the toxicity of methanol estrak Gelidium sp. higher. Differences in phytochemical test results and GC-MS are based on the percentage level of the compound in the material, so it is likely that compounds with a small percentage will not be detected in the phytochemical test.

Seaweed is one of the natural ingredients that have a variety of contents and sources of secondary metabolite compounds that have toxic activity. Toxic activity derived from a substance can be further developed and will provide benefits in the field of food and pharmacology. The presence of seaweed Glacilaria sp. and Gelidium sp. in the coastal area of Kalianda waters, South Lampung is quite abundant so that due to the availability of the abundance of these materials can be made of great potential to be utilized as a medicine. The purpose of this study was to analyze the level of toxicity using stratified estraction with n-hexane and methanol solvents and analyze chemical components with GC-MS based on the highest level of toxicity. The study was conducted from November to December 2021. Samples of Glacilaria sp. and Gelidium sp. taken from the waters of Kalianda, South Lampung. Furthermore, the samples were tested for toxicity by Brine Shrimp Lethality Test, phytochemical analysis, and GC-MS analysis. Based on the toxicity test results, the value of lc50 estrak n-hexane Glacilaria sp. 8446 µg/mL, methanol Glacilaria sp. 694 µg/mL n-hexane Gelidium sp. µg/mL and methanol gelidium sp. it amounted to 339 µg/mL. Phytochemical test results contain alkaloids, flavonoids, steroids and saponins while GC-MS test results contain steroids, flavonoids and diterpenoids. Differences in the level of toxicity of n-hexane and methanol in sebabakan by the nature of the compound so that the toxicity of methanol estrak Gelidium sp. higher. Differences in phytochemical test results and GC-MS are based on the percentage level of the compound in the material, so it is likely that compounds with a small percentage will not be detected in the phytochemical test.

Seaweed is one of the natural ingredients that have a variety of contents and sources of secondary metabolite compounds that have toxic activity. Toxic activity derived from a substance can be further developed and will provide benefits in the field of food and pharmacology. The presence of seaweed Glacilaria sp. and Gelidium sp. in the coastal area of Kalianda waters, South Lampung is quite abundant so that due to the availability of the abundance of these materials can be made of great potential to be utilized as a medicine. The purpose of this study was to analyze the level of toxicity using stratified estraction with n-hexane and methanol solvents and analyze chemical components with GC-MS based on the highest level of toxicity. The study was conducted from November to December 2021. Samples of Glacilaria sp. and Gelidium sp. taken from the waters of Kalianda, South Lampung. Furthermore, the samples were tested for toxicity by Brine Shrimp Lethality Test, phytochemical analysis, and GC-MS analysis. Based on the toxicity test results, the value of lc50 estrak n-hexane Glacilaria sp. 8446 µg/mL, methanol Glacilaria sp. 694 µg/mL n-hexane Gelidium sp. µg/mL and methanol gelidium sp. it amounted to 339 µg/mL. Phytochemical test results contain alkaloids, flavonoids, steroids and saponins while GC-MS test results contain steroids, flavonoids and diterpenoids. Differences in the level of toxicity of n-hexane and methanol in sebabakan by the nature of the compound so that the toxicity of methanol estrak Gelidium sp. higher. Differences in phytochemical test results and GC-MS are based on the percentage level of the compound in the material, so it is likely that compounds with a small percentage will not be detected in the phytochemical test.

Seaweed is one of the natural ingredients that have a variety of contents and sources of secondary metabolite compounds that have toxic activity. Toxic activity derived from a substance can be further developed and will provide benefits in the field of food and pharmacology. The presence of seaweed Glacilaria sp. and Gelidium sp. in the coastal area of Kalianda waters, South Lampung is quite abundant so that due to the availability of the abundance of these materials can be made of great potential to be utilized as a medicine. The purpose of this study was to analyze the level of toxicity using stratified estraction with n-hexane and methanol solvents and analyze chemical components with GC-MS based on the highest level of toxicity. The study was conducted from November to December 2021. Samples of Glacilaria sp. and Gelidium sp. taken from the waters of Kalianda, South Lampung. Furthermore, the samples were tested for toxicity by Brine Shrimp Lethality Test, phytochemical analysis, and GC-MS analysis. Based on the toxicity test results, the value of lc50 estrak n-hexane Glacilaria sp. 8446 µg/mL, methanol Glacilaria sp. 694 µg/mL n-hexane Gelidium sp. µg/mL and methanol gelidium sp. it amounted to 339 µg/mL. Phytochemical test results contain alkaloids, flavonoids, steroids and saponins while GC-MS test results contain steroids, flavonoids and diterpenoids. Differences in the level of toxicity of n-hexane and methanol in sebabakan by the nature of the compound so that the toxicity of methanol estrak Gelidium sp. higher. Differences in phytochemical test results and GC-MS are based on the percentage level of the compound in the material, so it is likely that compounds with a small percentage will not be detected in the phytochemical test.